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Wednesday, February 8 • 10:40am - 11:00am
Technical Session. RNA-Seq Based Transcriptome Analysis of White-tailed Deer (Odocoileus virginianus)

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AUTHORS: Emma K. Trone*, Christopher N. Jacques, James T. Lamer - Western Illinois University; Jun Wang, Guoqing Lu, Jun Wang - University of Nebraska-Omaha; Paul A. Shelton, Illinois Department of Natural Resources

ABSTRACT: White-tailed deer (Odocoileus virginianus; WTD) are popular game species found throughout North America and as such, have been widely studied, however, a paucity of genetic information exists at genome and transcriptome levels. This lack of basic genetic information leads to difficulties in answering questions that require comprehensive genetic backgrounds of a species or individual (i.e., studies focused on epidemiology or differential gene expression on an organismal scale). We sequenced RNA from 10 white-tailed deer livers and retropharyngeal lymph nodes using RNA-Seq. Specifically, we used the Illumina HiSeq 2500 Sequencing System (Illumina Inc., San Diego, CA, USA) with a HiSeq SBS sequencing kit version 4Transcriptomes were assembled paired-end using Trinity version 2.06. A total of 488,145,350 (243,310,654 from liver and 244,834,696 from RPLN) clean pair-end reads were generated and sequences ranged in size from 320bp to 25,965bp. When compared to protein databases, 51,647 transcripts were assigned genes in NCBI-NR and 47,292 transcripts were assigned genes in Uniprot. Gene Ontology analysis showed transcripts successfully mapped to 20 GO biological processes, 11 cellular components, and 11 molecular functions. The top 10 KEGG pathways include purine metabolism, biosynthesis of antibiotics, pyrimidine metabolism, glycerophospholipid metabolism, phosphatidylinositol signaling system, lysine degradation, aminoacyl-tRNA biosynthesis, glycolysis/glucogenesis, gluthaione metabolism, and drug metabolism- cytochrome P450. To our knowledge, this is only the second study to describe a WTD transcriptome and the third publication of WTD transcriptome data. By describing WTD transcriptomes, we are facilitating future in-depth white-tailed deer genetic studies.

Wednesday February 8, 2017 10:40am - 11:00am CST
Grand Ballroom C

Attendees (3)